Hello,
I am starting a project on which I would like to detect viral RNA (herpes) in human tissue. Essentially I would extract the RNA, perform targeted amplification and library prep for multiple viruses and then sequence. I think I have an idea of the bioinformatic system to use.
However, I am not really sure where to start on the library prep issue (i.e. which library prep system to use). I've looked into ScripSeq which sounded promising but seems very expensive.
Is there a "custom" protocol/template out there than I can use to accomplish these goals. My main concerns are (if i would to start with known herpes primers, how would I successfully make a library that contain adapters and index for multiplex?)
Thank you and excuse my naive questions. I am not new to using NGS for already commercially prepared custom gene panels (i.e. Qiagen targeted RNASeq panels) but I am new in developing custom assays so your help would be greatly appreciated.
I am starting a project on which I would like to detect viral RNA (herpes) in human tissue. Essentially I would extract the RNA, perform targeted amplification and library prep for multiple viruses and then sequence. I think I have an idea of the bioinformatic system to use.
However, I am not really sure where to start on the library prep issue (i.e. which library prep system to use). I've looked into ScripSeq which sounded promising but seems very expensive.
Is there a "custom" protocol/template out there than I can use to accomplish these goals. My main concerns are (if i would to start with known herpes primers, how would I successfully make a library that contain adapters and index for multiplex?)
Thank you and excuse my naive questions. I am not new to using NGS for already commercially prepared custom gene panels (i.e. Qiagen targeted RNASeq panels) but I am new in developing custom assays so your help would be greatly appreciated.
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