Hi ,
I am new to NGS analysis and trying my hand at fastx toolkit.I am trying to assess the quality of my sequence and part of the result is below.I would like to know the following
1.should we consider the mean column for assessing the quality of each cycle?
2.what is the minimum value below which the quality is considered bad?is it 20?
column count min max sum mean
1 10357317 2 40 404977359 39.1
2 10357317 2 40 404775168 39.08
3 10357317 2 40 404764272 39.08
your help will be greatly appreciated!!
Thanks,
Joji
I am new to NGS analysis and trying my hand at fastx toolkit.I am trying to assess the quality of my sequence and part of the result is below.I would like to know the following
1.should we consider the mean column for assessing the quality of each cycle?
2.what is the minimum value below which the quality is considered bad?is it 20?
column count min max sum mean
1 10357317 2 40 404977359 39.1
2 10357317 2 40 404775168 39.08
3 10357317 2 40 404764272 39.08
your help will be greatly appreciated!!
Thanks,
Joji
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