When I was assembling the reads , I found this scenario:
TAA-CCTCCCCC-AAANTT-CAGA Consensus
TAA-CCTCCCCC-AAACTT
TAA-CCTCCCCC-AAACTTACAGA
TAA-CCT-CCCC-AAACTTACAGA
TAA-CCTCCCCCAAAACTT-CAGA
TAACCCTCCCCC-AAAATT-CAGA
TAA-CCTCCCCC-AAAATT-CAGA
TAA-CCTCCCCA-AAACTTACAGA
TAA-CCTCCCCC-AAAATT-CAGA
TAA-CCTCCCCC-AAAATT-CAGA
---A-CCTCCCCC-AAAATT-CAGA
The first line is the consensus sequence. You can find a N.
Which was caused by 5C and 5A mapped to that position.
Someone told me this was caused by the homopolymer, the
C observed at the position is likely to be one part of the homopolymer
ahead. Have you met this problem before? Do you think it is possible?
TAA-CCTCCCCC-AAANTT-CAGA Consensus
TAA-CCTCCCCC-AAACTT
TAA-CCTCCCCC-AAACTTACAGA
TAA-CCT-CCCC-AAACTTACAGA
TAA-CCTCCCCCAAAACTT-CAGA
TAACCCTCCCCC-AAAATT-CAGA
TAA-CCTCCCCC-AAAATT-CAGA
TAA-CCTCCCCA-AAACTTACAGA
TAA-CCTCCCCC-AAAATT-CAGA
TAA-CCTCCCCC-AAAATT-CAGA
---A-CCTCCCCC-AAAATT-CAGA
The first line is the consensus sequence. You can find a N.
Which was caused by 5C and 5A mapped to that position.
Someone told me this was caused by the homopolymer, the
C observed at the position is likely to be one part of the homopolymer
ahead. Have you met this problem before? Do you think it is possible?
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