Dear All,
I am currently working on 454 sequencing data. The average length of reads is ~200bp, and coverage is ~8-10X.
Does anybody tell me how the data coverage is? Is it ok or not?
Because when I do annotation, I found many many single nucleotide changes (mutation/insertion/deletion).
Thank you.
I am currently working on 454 sequencing data. The average length of reads is ~200bp, and coverage is ~8-10X.
Does anybody tell me how the data coverage is? Is it ok or not?
Because when I do annotation, I found many many single nucleotide changes (mutation/insertion/deletion).
Thank you.
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