Hi all,
I am sorry if my question sould sound stupid but I am rather new to NGS technologies and illumina platform
We are going to perform a micorbial community ecology study. We have a set of 48 samples that should be selectively amplified for variable 16S DNA regions.
Could anyone have suggest a suitable and cheap solution for this kind of amplicon sequencing approach?
I would like to reduce the number of libraries by multiplexing indexed samples, but many protocols are described in literature... Which one is the best solution for you own experiences?
Thanks a lot in advance,
Gian
I am sorry if my question sould sound stupid but I am rather new to NGS technologies and illumina platform
We are going to perform a micorbial community ecology study. We have a set of 48 samples that should be selectively amplified for variable 16S DNA regions.
Could anyone have suggest a suitable and cheap solution for this kind of amplicon sequencing approach?
I would like to reduce the number of libraries by multiplexing indexed samples, but many protocols are described in literature... Which one is the best solution for you own experiences?
Thanks a lot in advance,
Gian
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