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  • Problem of unmapped reads

    Dear all,
    I have problem to align my RNA-seq experiments. FAstq show not low quality but however I have very low allign 50%. How can understand if the problem are related with some contaminants (?) or to my gtf file?
    RNA seq 2x100 PE from FPE samples I allign using topath hg19 ( 60ML reads).

    Exist a way to check the quality of my library after allignmen (complexity?)
    thanks for your help!

  • #2
    You can use samtools idxstats, but it is hard to tell what would be wrong (contamination or wrong annotation).

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