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Hi!
I am a undergraduate student and planning to conduct single-embryo RNA-seq.
The developmental stage of samples are various, from blastula (about 1k cells) to just before hatching.
(I am using zebrafish, X.laevis and chick.)
Could I make cDNA libraries in the same way?
Some members in my lab are performing Quartz-seq, so there are necessary reagents.
What I would like to know is…
1. Whether I could perform Quartz-seq with late stage embryo.
(too many impurities, or RNAs?)
2. or, could I make cDNA libraries without amplification?
(especially eary stage embryo)
If I should verify myself, I would be glad to know how to do.
thank you very much.
I am a undergraduate student and planning to conduct single-embryo RNA-seq.
The developmental stage of samples are various, from blastula (about 1k cells) to just before hatching.
(I am using zebrafish, X.laevis and chick.)
Could I make cDNA libraries in the same way?
Some members in my lab are performing Quartz-seq, so there are necessary reagents.
What I would like to know is…
1. Whether I could perform Quartz-seq with late stage embryo.
(too many impurities, or RNAs?)
2. or, could I make cDNA libraries without amplification?
(especially eary stage embryo)
If I should verify myself, I would be glad to know how to do.
thank you very much.