Hi everyone,
I am new to sequencing and am wondering if it would be possible to perform de novo assembly using MiSeq, for a 35 MB fungal genome ?
Technically speaking, how could we increase the coverage with the Nextera kit, already purchased for this purpose ?
Is it feasible to run the same sample many times to increase the fastq file, I mean the ouput ?
Sorry, if I am saying stupid things but this is so important to me,
Thank you !
I am new to sequencing and am wondering if it would be possible to perform de novo assembly using MiSeq, for a 35 MB fungal genome ?
Technically speaking, how could we increase the coverage with the Nextera kit, already purchased for this purpose ?
Is it feasible to run the same sample many times to increase the fastq file, I mean the ouput ?
Sorry, if I am saying stupid things but this is so important to me,
Thank you !
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