Dear all,
I'm trying to import MiSeq FastQ files to CLC Genomics Workbench (version 4 point something), and I want to make sure that I'm doing the right thing. They're mate-pair data, so I choose mate-pair in the paired read information option. However, I'm not sure about the "Minimum" and "Maximum distance". How can I know the right values to enter?
The sequencing technician told me that the read length is 250 bp and the insert size is 2.9 kb. Do these values have anything to do with the minimum and maximum distance of the mate-pair information?
Your help is really appreciated.
I'm trying to import MiSeq FastQ files to CLC Genomics Workbench (version 4 point something), and I want to make sure that I'm doing the right thing. They're mate-pair data, so I choose mate-pair in the paired read information option. However, I'm not sure about the "Minimum" and "Maximum distance". How can I know the right values to enter?
The sequencing technician told me that the read length is 250 bp and the insert size is 2.9 kb. Do these values have anything to do with the minimum and maximum distance of the mate-pair information?
Your help is really appreciated.
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