Hello!
I assigne K0 onthology to my genes from bacteria metatranscriptome. Now I'd like to carry out a differential expression analysis. Obviously, the same K0 can be involved in multiple pathways and metabolisms.
How do you deal with this in pathway analysis? I mean, if I want to test if I have enrichment of certain pathways or metabolisms, how should I consider each gene (belonging to which pathway or metabolism)?
sorry for the basic question, but I'm really newbie!
Thanks
I assigne K0 onthology to my genes from bacteria metatranscriptome. Now I'd like to carry out a differential expression analysis. Obviously, the same K0 can be involved in multiple pathways and metabolisms.
How do you deal with this in pathway analysis? I mean, if I want to test if I have enrichment of certain pathways or metabolisms, how should I consider each gene (belonging to which pathway or metabolism)?
sorry for the basic question, but I'm really newbie!
Thanks
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