Hi everybody,
I have six samples for RNA-Seq. 3 WT and 3 mutants.
I think the amount of RNA used for each library construction might vary.
If people loaded a bit more RNA for one sample, there will be more reads in this sample.
As we know, for qPCR, we use some genes (GAPDH...) as internal control.
My question is how cuffidiff normalized this variation in the amount of RNA used for RNA-seq??
Looking forward to hearing from you.
Jiwen
I have six samples for RNA-Seq. 3 WT and 3 mutants.
I think the amount of RNA used for each library construction might vary.
If people loaded a bit more RNA for one sample, there will be more reads in this sample.
As we know, for qPCR, we use some genes (GAPDH...) as internal control.
My question is how cuffidiff normalized this variation in the amount of RNA used for RNA-seq??
Looking forward to hearing from you.
Jiwen
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