Hi all,
I made cDNA libaries using the Clontech SMART-Seq v4 ultra low RNA kit and made Nextera XT sequencing libraries from that cDNA. The bioanalyzer result (DNA HS) for the Nextera XT libraries is attached. Obviously Anamenia failed but I'm debating whether the others are OK for pooled sequencing. There is a really large variation in size range. Any input would be very welcome.
Thanks,
Kevin
I made cDNA libaries using the Clontech SMART-Seq v4 ultra low RNA kit and made Nextera XT sequencing libraries from that cDNA. The bioanalyzer result (DNA HS) for the Nextera XT libraries is attached. Obviously Anamenia failed but I'm debating whether the others are OK for pooled sequencing. There is a really large variation in size range. Any input would be very welcome.
Thanks,
Kevin
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