Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • sff_extract error

    Hello,

    sorry I am new in this I tried to convert sff file to fasta and fastq files. Firstly I installed python 2.7.6 then biopython 1.65 and finally seq_crumbs 0.1.9 and i tried sff_extract but there is a problem :

    ]# sff_extract IXXLJHH01.sff
    Traceback (most recent call last):
    File "/usr/bin/sff_extract", line 25, in <module>
    from crumbs.seqio import write_seqs
    File "/usr/lib/python2.6/site-packages/crumbs/seqio.py", line 35, in <module>
    from crumbs.utils.file_formats import get_format, peek_chunk_from_file
    File "/usr/lib/python2.6/site-packages/crumbs/utils/file_formats.py", line 22, in <module>
    from crumbs.settings import get_setting
    File "/usr/lib/python2.6/site-packages/crumbs/settings.py", line 133, in <module>
    _settings = _Settings()
    File "/usr/lib/python2.6/site-packages/crumbs/settings.py", line 114, in __init__
    self.load_settings()
    File "/usr/lib/python2.6/site-packages/crumbs/settings.py", line 119, in load_settings
    for key, val in globals().viewitems():
    AttributeError: 'dict' object has no attribute 'viewitems'

    What should I do for this?
    Thanks,

  • #2
    Does seq_crumbs require python 2.7.x? Looks like your software got installed in python2.6.

    Comment


    • #3
      Yes, you are right. However, I installed 2.7.6 how can i change it do you have any idea?

      Comment


      • #4
        Use one of the options here to see if you can set your default python version to 2.7. You may be able to use crumbs installed in 2.6 but the other thing to try would be to re-do the biopython/crumbs install so they would install under 2.7.x.

        Comment


        • #5
          Thank you!

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Essential Discoveries and Tools in Epitranscriptomics
            by seqadmin


            The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
            Yesterday, 07:01 AM
          • seqadmin
            Current Approaches to Protein Sequencing
            by seqadmin


            Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
            04-04-2024, 04:25 PM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 04-11-2024, 12:08 PM
          0 responses
          43 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 10:19 PM
          0 responses
          43 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 09:21 AM
          0 responses
          38 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-04-2024, 09:00 AM
          0 responses
          55 views
          0 likes
          Last Post seqadmin  
          Working...
          X