Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
how to use mira to assemble the fastq generated by 454 sequencing dingkai0564 Bioinformatics 6 05-26-2013 01:12 PM
Using Mosaik to assemble bacterial genome 454 sequencing jpearl01 Bioinformatics 2 03-27-2013 06:29 AM
MIRA assembly with MID barcodes for 454? raw937 Bioinformatics 4 06-14-2011 11:54 PM
MIRA assembly with MID barcodes for 454? raw937 454 Pyrosequencing 1 06-13-2011 11:52 AM
Using PHRAP to assemble 454 contigs and Sanger reads cleoho175 Bioinformatics 8 11-24-2010 11:45 AM

Thread Tools
Old 08-31-2011, 09:25 PM   #1
Location: lasvegas

Join Date: Nov 2010
Posts: 10
Red face How to use MIRA to assemble 454 fastaq file alone


I have some questions to use MIRA: I am asking for help about how to use the mira to assemble my fastq file

I had downloaded the fastq file I am interested from NCBI SRA database. But It only provides fastq file. When I read the manual of Mira,

it says another XML file which extracted from the original SFF file to indicate the vector or adaptor information is needed. Since, I only got the fastq file from the internet,can I do the assmble work alone using the fastq file without that XML file.
dingkai0564 is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:26 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO