Sorry for the long Title!
We have a de novo assembled transcriptome and have become aware that a portion of the transcripts are in fact different portions of the same locus (sometimes with a short region of overlap but often not). Im sure this is common but its rarely commented on. I was wondering if there was a way to use a distant (several millions of years) but very well annotated transcriptome (e.g. Arabidopsis) to pair up transcripts that seem to come from the same locus. One concern is that gene duplication etc might mean that the results arent very reliable... but Id like to give it a try!
Thanks a lot!
Mark
We have a de novo assembled transcriptome and have become aware that a portion of the transcripts are in fact different portions of the same locus (sometimes with a short region of overlap but often not). Im sure this is common but its rarely commented on. I was wondering if there was a way to use a distant (several millions of years) but very well annotated transcriptome (e.g. Arabidopsis) to pair up transcripts that seem to come from the same locus. One concern is that gene duplication etc might mean that the results arent very reliable... but Id like to give it a try!
Thanks a lot!
Mark
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