Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Questions on Agilent SureSelect Indexing Kit

    Hi,

    does anybody have details on the new SureSelect Indexing Kits?
    I am interested in targeting 12 samples and pool and sequence them on 1 lane of Illumina GAII.

    My question is:

    - What is the maximum amount of DNA that I can target using a 55k baits SureSelect library? For one sample with a Paired-End approach one can target up to 6.6Mb. Do I have to divide this amount by the number of samples that I want to target (i.e. 6.6/12=0.55Mb max per sample)?


    Thanks!
    if1
    Last edited by if1; 05-17-2010, 08:16 AM. Reason: typo

  • #2
    IMHO, indexing do not influence the amount of DNA you can capture with one SureSelect library. There is still one library for the enrichment of one DNA sample. Usage of indexing adapters and primers only allows you to sequence up to 12 enriched samples on a single GAII lane. Unfortunately it do not allow you to enrich 12 samples using a single SureSelect library...

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    7 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    7 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    49 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    66 views
    0 likes
    Last Post seqadmin  
    Working...
    X