Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
EdgeR test for differential expression. tirohia Bioinformatics 0 06-17-2015 12:54 AM

Thread Tools
Old 05-26-2017, 03:12 AM   #1
Junior Member

Join Date: May 2016
Posts: 2
Default Differential expression of miRNAs using edgeR

I am working on elucidating pathologically relevant miRNAs responsible for disease progression and chemoresistance in chronic lymphocytic leukemia. For differential expression of miRNAs I am using the edgeR package. I am new to statistics, edger and any help would be highly appreciated. The samples are classified as 1) Indolent which has 7 patient samples 2) Chemo-responsive, 9 patient samples 3) Non-responsive, 7 patient samples 4) Control, 2 samples.
I want to check the differential expression of miRNAs as follows:

For comparing two groups like indolent vs chemo-responsive contrast matrix contrast(-1,1,0,0) works well. What should be the contrast matrix when comparing:

(a) Control vs Indolent,Chemo-responsive,Non-responsive
(b) Indolent vs Chemo-responsive,Non-responsive.
Kamy24 is offline   Reply With Quote

edger, glmlrt

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 09:19 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO