Hi,
I'm wondering if anyone else has obtained a result like this in a library construction. Also, a target of this post is the Epicentre tech assist.
I've been contructing a library with ScripSeq Library Preparation kit (Illumina-compatible) from Epicentre. The starting material was rRNA depleted (from Trizol total RNA extraction). The initial RNA and rRNA depleted sample looks good in the bioanalyzer. But after following the protocol and purify the library with XP beads (I also tried MinElute columns, with same result but a lot of primer-dimers), the library pattern does not look good. Attached is the bioanalizer trace from one of the samples (with 15 cycles in the PCR amplification step, having already used between 10-15).
Thanks
I'm wondering if anyone else has obtained a result like this in a library construction. Also, a target of this post is the Epicentre tech assist.
I've been contructing a library with ScripSeq Library Preparation kit (Illumina-compatible) from Epicentre. The starting material was rRNA depleted (from Trizol total RNA extraction). The initial RNA and rRNA depleted sample looks good in the bioanalyzer. But after following the protocol and purify the library with XP beads (I also tried MinElute columns, with same result but a lot of primer-dimers), the library pattern does not look good. Attached is the bioanalizer trace from one of the samples (with 15 cycles in the PCR amplification step, having already used between 10-15).
Thanks
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