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Old 05-26-2017, 06:30 AM   #1
seqtechno1
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Default bluepippin size selection for SMRTbells

Hello,

at which step of the 10kb protocol for preparation of SMRTbell libraries do you run BluePippin 20kb cassette for size selection? I am preparing gDNAs for complete sequencing.
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Old 05-26-2017, 03:44 PM   #2
nucacidhunter
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Current 10kb protocol does not include a Pippin size selection step but if you want to include it then it will be after ligation clean ups. You might follow a Sequel 10 and 20kb hybrid protocol.
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Old 06-18-2017, 11:33 PM   #3
weigrc
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With PacBio 10kb protocol, we got the library sizing around 7kb on agarose gel, no need to do size selection.
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