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Old 04-15-2011, 02:33 AM   #1
oceanxie
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Location: Shanghai,China

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Smile how to evaluate raw data

Hey, i'm a layman in Bioinformatics. Our lab have completed a project of mRNA-sequencing on illumina platform and obtained about 30 millions 150bp-200bp reads. The sample is a mouse cell line.
i have some questions. for example,how to evaluate the raw data? is there any way to check the qulity of raw data, good or bad?
thank you for help. Best Wishes.
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Old 04-15-2011, 02:52 AM   #2
boetsie
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you might try fastQC which does a quality control on raw illumina data;

http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/
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