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  • pacBioToCA fails with single-end data

    Hello everyone,

    I am trying to correct my pacbio reads with unmated solexa reads. I use the program pacbioToCA for this. After an hour I retrieve the error:
    ----------------------------------------START CONCURRENT Sat Jan 11 00:36:54 2014
    /home/jaco001/fungiAssembly/data//tempmadurellaPacbio/runPartition.sh 1
    No contig account found in bank /home/jaco001/fungiAssembly/data//tempmadurellaPacbio/asm.bnk_partition1.bnk
    ----------------------------------------END CONCURRENT Sat Jan 11 02:41:17 2014 (7463 seconds)
    Failed to run correction job 1. Remove /home/jaco001/fungiAssembly/data//tempmadurellaPacbio/runPartition.sh to try again.
    When searching on this error, I found another Thread. In this thread is mentioned there may be a problem with paired end data. I used this frg file:
    {VER
    ver:2
    }
    {LIB
    act:A
    acc:madurellaIllumina
    ori:U
    mea:0.000
    std:0.000
    src:
    .
    nft:20
    fea:
    forceBOGunitigger=0
    isNotRandom=0
    doNotTrustHomopolymerRuns=0
    doTrim_initialNone=0
    doTrim_initialMerBased=0
    doTrim_initialFlowBased=0
    doTrim_initialQualityBased=0
    doRemoveDuplicateReads=0
    doTrim_finalLargestCovered=0
    doTrim_finalEvidenceBased=0
    doTrim_finalBestEdge=0
    doRemoveSpurReads=0
    doRemoveChimericReads=0
    doCheckForSubReads=0
    doConsensusCorrection=0
    forceShortReadFormat=0
    constantInsertSize=0
    fastqQualityValues=solexa
    fastqOrientation=innie
    fastqReads=/home/jaco001/fungiAssembly/data/IlluminaNoNegs.fq
    .
    }
    {VER
    ver:1
    }
    The orientation is unmated (ori=U), so I said to the program my input is unmated. Does someone know how I can solve this problem?

  • #2
    Did you by any chance solve this problem? I run into the same problem while doing self-correction , so no other platform reads are involved.

    Comment


    • #3
      I did not solve this problem...

      Comment

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