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  • strange bwa/samtools behaviour (?)

    Hello everyone
    First of all, I'd like to thank you for this super-useful community everyone's contributing to build up. It's a kind of next-gen sequencing for me
    Now, coming to my question:
    I'm quite new in next-gen sequencing and I'm working with Illumina data (paired-end, 76bp long reads, whole exome) and I cannot have any of my aligned bam/sam files with header included. That is, I have all the pretty @SQ lines and @PG line at the end of the header. What I can't gain is the @HD/tVN/SO line at the very beginning of the header.

    In addiction, if i try to do
    bwa aln - bwa sampe - samtools view - samtools sort in a separate way, when I try to give a look to my new-beautiful-bam-file's header I'll receive always this message:

    samtools view -H prova.bam <- my command
    [bam_header_read] EOF marker is absent.

    while, when I sort it, I get this other one:
    samtools sort prova.bam prova_sorted <- my command

    [bam_header_read] EOF marker is absent. The input is probably truncated.

    All of this doesn't happen at all if I do that in a piped way...even if the @HD/tVN:/tSO line is still missing

    Any ideas would be particularly appreciated
    Last edited by francesconea; 04-12-2011, 05:16 AM. Reason: wanted to better check posting options
    wherever you go, whatever you do, always bring a bioinformatic with you

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