I try to sequence multiplex (21 samples) amplicons for months !! Amplicon size is around 609 pb (with MID ...). The problem is that the yield is very low (2%) with 2 or 4 cpb and when I sequence I juste have 50 bp instead of 609 pb (bioanalyzer is ok, no small fragments).
Do you think to increase elongation time of emPCR will be better or the problem come from an other step ?
Thank for your help !
Do you think to increase elongation time of emPCR will be better or the problem come from an other step ?
Thank for your help !
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