Does anyone know of any good methods for multiplexing more than 384 samples on a MiSeq run? The Nextera XT Index Kit allows indexing for a maximum of 384 samples. We have already purchased one of these kits, but would like to add more samples onto our run. I know the Illumina customer letter provided sequences for some of their barcodes, but not for all of them. Does anyone know if the rest of these are available elsewhere? I suppose it would be possible to order oligos identical to the Nextera barcode adaptors but with different 8 bp barcode sequences. Any thoughts?
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
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Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...-
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