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  • DNA Methylation Data Analysis Workshop - 2-5 November 2020 in Berlin



    How to use bisulfite-treated sequencing to study DNA methylation
    Advance your epigenetics research with NGS methods.

    Link to workshop website

    In a nutshell
    • Learn how bisulfite sequencing works
    • Understand how bisulfite-treated reads are mapped to a reference genome
    • Perform basic analyses (call methylated regions, perform basic downstream analyses)
    • Use shell scripting to create reusable data pipelines
    • Visualize results (ready-to-publish)


    The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

    By the end of this workshop the participants will:
    • be familiar with the sequencing method of Illumina
    • understand how bisulfite sequencing works
    • be aware of the mapping problem of bisulfite-treated data
    • understand how bisulfite-treated reads are mapped to a reference genome
    • be familiar with common data formats and standards
    • know relevant tools for data processing
    • automate tasks with shell scripting to create reusable data pipelines
    • perform basic analyses (call methylated regions, perform basic downstream analyses)
    • plot and visualize results (ready-to-publish)
    • be able to reuse all analyses


    More information here
    ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS).

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