Hi everyone.
I'm quite new to NGS and is currently working on creating a custom database of reference genomes.
I want to check if theres any human contamination in the database and therefore I'm trying to map the database to the human genome (GRCh38).
As I understand it, i have to create pseudoreads/contigs from each genome in my database before I can map them to the human genome.
Is that the right way to proceed? And if so, is there a smart way of doing this?
Any help is really appreciated
I'm quite new to NGS and is currently working on creating a custom database of reference genomes.
I want to check if theres any human contamination in the database and therefore I'm trying to map the database to the human genome (GRCh38).
As I understand it, i have to create pseudoreads/contigs from each genome in my database before I can map them to the human genome.
Is that the right way to proceed? And if so, is there a smart way of doing this?
Any help is really appreciated
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