Hello,
My question is:
How long can RNA-seq libraries be frozen at -20C after initial sequencing? Can a frozen RNA-seq library be re-sequenced 6 months later with high quality?
I spoke to someone at our local sequencing facility, and she indicated that she has frozen libraries and re-sequenced them 3 months later with no problems. Does anyone know if a library can be frozen for 6-12 months?
Background information:
Our lab is going to conduct a large amount of RNA seq experiments (we have close to 100 samples, including replicates). However, we cannot afford to sequence all of our samples deeply.
We want to sequence one replicate per condition (10-15 million reads) to see where interesting changes are occuring. We would then re-sequence those interesting conditions with replicates (50 million reads each).
However, we want our initial libraries frozen for later re-sequencing, to avoid paying for extra library preps and sequenced reads.
My question is:
How long can RNA-seq libraries be frozen at -20C after initial sequencing? Can a frozen RNA-seq library be re-sequenced 6 months later with high quality?
I spoke to someone at our local sequencing facility, and she indicated that she has frozen libraries and re-sequenced them 3 months later with no problems. Does anyone know if a library can be frozen for 6-12 months?
Background information:
Our lab is going to conduct a large amount of RNA seq experiments (we have close to 100 samples, including replicates). However, we cannot afford to sequence all of our samples deeply.
We want to sequence one replicate per condition (10-15 million reads) to see where interesting changes are occuring. We would then re-sequence those interesting conditions with replicates (50 million reads each).
However, we want our initial libraries frozen for later re-sequencing, to avoid paying for extra library preps and sequenced reads.
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