Tweet
I need help. How to remove N between in sequences using by velvet?
-
-
Hi i am about to ask the same questions. I had some the NNNN between my sequences and I had done some PCR to close the gaps. In this case do anyone know what type of software to remove the NNNN and replce it with new sequences?
Thanks -
You can avoid the N's in the velvet output by using the -scaffold option when running velvetg.
Best regards,
Peter
Originally posted by chronix View PostComment
-
One solution could be to use the obtained sequence together with velvet using the -long option.
Originally posted by Rachel View PostComment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment