I recently submitted the GecKO library (~120K guides) for a MiSeq Nano run to check diversity (and mostly to check my custom seq primer etc. was working). It performed 1.3M reads, so about 10x the library size. I realize this is not nearly enough, since it's recommended to do 100x the library size. Even with 1/10th of the recommended depth, I had about 2.5% unread guides (rec is <0.5%). Do you think I can go ahead with this library? I really don't want to spend more money to do a 12M+ run. Thoughts?
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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03-22-2024, 06:39 AM -
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