hi..
I am working on small rna sequencing which was sequenced through illumina hiseq. the read length i got was 30bp. When i removed 3' adapter, i see that 90% of the reads are having 30bp. I tried with cutadapt, fastq-mcf tools but the result didnt change, peaking at 30bp. The adapter i got is correct adapter according to sequencing lab. When i look at other papers, everyone report that the peak is around 21- 24 bp.. can anyone suggest what steps i can do to process my data?
I am working on small rna sequencing which was sequenced through illumina hiseq. the read length i got was 30bp. When i removed 3' adapter, i see that 90% of the reads are having 30bp. I tried with cutadapt, fastq-mcf tools but the result didnt change, peaking at 30bp. The adapter i got is correct adapter according to sequencing lab. When i look at other papers, everyone report that the peak is around 21- 24 bp.. can anyone suggest what steps i can do to process my data?
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