how high-through sequencing detect I? as G ?

zslee · 01-14-2010, 10:02 PM

i am doing rna editing analysis using solexa data,
i wann know which base is a I detected as ? even though it's translated as G in vivo
thanks

elaney_k · 01-15-2010, 09:52 AM

I'm not sure if I understand your question, however for detection of A to I RNA editing you need to sequence both the genomic DNA and RNA. If editing occurs you will only see an "A" in the DNA sequence but at the same genomic position in the cDNA (RNA) you will see both As and Gs.
Have a look at this publication for more info:
"Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing" Science. 2009 May 29;324(5931):1210-3.

zslee · 01-15-2010, 05:04 PM

OK,thanks for you reply and sorry for my english

i understand ~~

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01-14-2010, 10:02 PM	#1
zslee Member Location: shanghai of china Join Date: May 2009 Posts: 29	how high-through sequencing detect I? as G ? i am doing rna editing analysis using solexa data, i wann know which base is a I detected as ? even though it's translated as G in vivo thanks

01-15-2010, 09:52 AM	#2
elaney_k Member Location: Dublin, Ireland Join Date: Mar 2008 Posts: 55	I'm not sure if I understand your question, however for detection of A to I RNA editing you need to sequence both the genomic DNA and RNA. If editing occurs you will only see an "A" in the DNA sequence but at the same genomic position in the cDNA (RNA) you will see both As and Gs. Have a look at this publication for more info: "Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing" Science. 2009 May 29;324(5931):1210-3.

01-15-2010, 05:04 PM	#3
zslee Member Location: shanghai of china Join Date: May 2009 Posts: 29	OK,thanks for you reply and sorry for my english i understand ~~