using bfast for mapping bacterial and viral genomes

kakar_nipun · 05-20-2010, 09:24 AM

Hi All

I was intending to map SOLID reads to the viral and bacterial genomes, and was thinking if i can use the same indices as mentioned in the Bfast manual, or do i need to create new ones?

After reading under advanced topics in the manual, i felt that using the same indices might just be stringent. Can someone throw some more light on this?

nilshomer · 05-20-2010, 11:37 AM

Quote:

Originally Posted by kakar_nipun

Hi All

I was intending to map SOLID reads to the viral and bacterial genomes, and was thinking if i can use the same indices as mentioned in the Bfast manual, or do i need to create new ones?

After reading under advanced topics in the manual, i felt that using the same indices might just be stringent. Can someone throw some more light on this?

They should work well for the viral and bacterial genomes.

drio · 05-20-2010, 12:14 PM

Quote:

Originally Posted by kakar_nipun

Hi All

I was intending to map SOLID reads to the viral and bacterial genomes, and was thinking if i can use the same indices as mentioned in the Bfast manual, or do i need to create new ones?

After reading under advanced topics in the manual, i felt that using the same indices might just be stringent. Can someone throw some more light on this?

There has been a lot of work behind the default indexes used for human (check support material from the paper). There is also code butil/btestindexes that
can help you test how they behave with different error rates, indels, etc... I would strongly suggest to create your own indexes (and compare with the default ones).

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05-20-2010, 09:24 AM	#1
kakar_nipun Junior Member Location: texas,us Join Date: Jul 2009 Posts: 3	using bfast for mapping bacterial and viral genomes Hi All I was intending to map SOLID reads to the viral and bacterial genomes, and was thinking if i can use the same indices as mentioned in the Bfast manual, or do i need to create new ones? After reading under advanced topics in the manual, i felt that using the same indices might just be stringent. Can someone throw some more light on this?