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Old 11-22-2017, 04:48 AM   #1
Junior Member
Location: USA

Join Date: Apr 2014
Posts: 4
Default alternative SPRI beads

Is anyone using SPRI beads other than Beckman Coulter's Agencourt AMPure XP? Is anyone using MagBio's HighPrep PCR beads?
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Old 11-22-2017, 04:35 PM   #2
Senior Member
Location: US

Join Date: Dec 2010
Posts: 452

Yep, we have been using MagBio's HighPrep PCR beads and the KapaPure beads. Both worked similar to the Ampure XP beads, also for size selections.
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Old 02-23-2018, 07:22 AM   #3
Location: college station

Join Date: Jan 2012
Posts: 22

We just follow the DIY SPRI bead protocol here We tested our home-brewed mixture with Ampure beads and didn't find much difference.

It is extremely cheap to make compared to the Ampure beads. You could make a liter of it for 400 bucks.
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Old 04-04-2018, 02:40 AM   #4
Location: Poland

Join Date: Feb 2014
Posts: 13

There is also a solution PCRClean Dx. Did anyone try this one?
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Old 04-13-2018, 04:07 AM   #5
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Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 208

we use SeraMag beads (carboxyl beads, hydrophylic). With a 150 EUR bottle (15 ml) one can make 750 ml of bead solution. We follow the protocol detailed in this paper: PMID:22267522
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Old 04-26-2018, 06:56 AM   #6
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Location: UK

Join Date: Apr 2018
Posts: 8

Also using homemade beads with the same protocol as melop, much cheaper.

If you're worried they aren't as good you can validate them against whatever you're using now.
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