alternative SPRI beads

aerseq · 11-22-2017, 04:48 AM

Is anyone using SPRI beads other than Beckman Coulter's Agencourt AMPure XP? Is anyone using MagBio's HighPrep PCR beads?

luc · 11-22-2017, 04:35 PM

Yep, we have been using MagBio's HighPrep PCR beads and the KapaPure beads. Both worked similar to the Ampure XP beads, also for size selections.

melop · 02-23-2018, 07:22 AM

We just follow the DIY SPRI bead protocol here https://openwetware.org/wiki/SPRI_bead_mix. We tested our home-brewed mixture with Ampure beads and didn't find much difference.

It is extremely cheap to make compared to the Ampure beads. You could make a liter of it for 400 bucks.

sequencingfan · 04-04-2018, 02:40 AM

There is also a solution PCRClean Dx. Did anyone try this one?

Simone78 · 04-13-2018, 04:07 AM

we use SeraMag beads (carboxyl beads, hydrophylic). With a 150 EUR bottle (15 ml) one can make 750 ml of bead solution. We follow the protocol detailed in this paper: PMID:22267522

NGSMicro · 04-26-2018, 06:56 AM

Also using homemade beads with the same protocol as melop, much cheaper.

If you're worried they aren't as good you can validate them against whatever you're using now.

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11-22-2017, 04:48 AM	#1
aerseq Junior Member Location: USA Join Date: Apr 2014 Posts: 4	alternative SPRI beads Is anyone using SPRI beads other than Beckman Coulter's Agencourt AMPure XP? Is anyone using MagBio's HighPrep PCR beads?

11-22-2017, 04:35 PM	#2
luc Senior Member Location: US Join Date: Dec 2010 Posts: 452	Yep, we have been using MagBio's HighPrep PCR beads and the KapaPure beads. Both worked similar to the Ampure XP beads, also for size selections.

02-23-2018, 07:22 AM	#3
melop Member Location: college station Join Date: Jan 2012 Posts: 22	We just follow the DIY SPRI bead protocol here https://openwetware.org/wiki/SPRI_bead_mix. We tested our home-brewed mixture with Ampure beads and didn't find much difference. It is extremely cheap to make compared to the Ampure beads. You could make a liter of it for 400 bucks.

04-04-2018, 02:40 AM	#4
sequencingfan Member Location: Poland Join Date: Feb 2014 Posts: 13	There is also a solution PCRClean Dx. Did anyone try this one?

04-13-2018, 04:07 AM	#5
Simone78 Senior Member Location: Basel (Switzerland) Join Date: Oct 2010 Posts: 208	we use SeraMag beads (carboxyl beads, hydrophylic). With a 150 EUR bottle (15 ml) one can make 750 ml of bead solution. We follow the protocol detailed in this paper: PMID:22267522

04-26-2018, 06:56 AM	#6
NGSMicro Junior Member Location: UK Join Date: Apr 2018 Posts: 8	Also using homemade beads with the same protocol as melop, much cheaper. If you're worried they aren't as good you can validate them against whatever you're using now.