Zero FPKM at some Cufflinks output file

fuad193 · 06-06-2012, 10:35 PM

Hi

Some transcripts at the final output of cufflinks have zero FPKM and coverage. I am using annotation from ensembl (annotation_ensembl.gtf). This is my command line:

cufflinks -p 8 -G annotation_ensembl.gtf -b genome.fa aln.sam.sorted

aln.sam is produced via bowtie and the aligned reads were 75%. I don't think the problem is in the sam file.

kopi-o · 06-07-2012, 12:16 AM

I don't think there is necessary any problem. Earlier versions of Cufflinks used to output only transcripts with non-zero FPKM, but later versions output values for all transcripts that you have provided. So whether you are genuinely having a problem would depend on which version you are using.

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06-06-2012, 10:35 PM	#1
fuad193 Member Location: N/A Join Date: Feb 2012 Posts: 17	Zero FPKM at some Cufflinks output file Hi Some transcripts at the final output of cufflinks have zero FPKM and coverage. I am using annotation from ensembl (annotation_ensembl.gtf). This is my command line: cufflinks -p 8 -G annotation_ensembl.gtf -b genome.fa aln.sam.sorted aln.sam is produced via bowtie and the aligned reads were 75%. I don't think the problem is in the sam file.

06-07-2012, 12:16 AM	#2
kopi-o Senior Member Location: Stockholm, Sweden Join Date: Feb 2008 Posts: 319	I don't think there is necessary any problem. Earlier versions of Cufflinks used to output only transcripts with non-zero FPKM, but later versions output values for all transcripts that you have provided. So whether you are genuinely having a problem would depend on which version you are using.