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10-01-2012, 01:00 PM
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#1 |
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Junior Member
Location: sf bay area Join Date: Oct 2010
Posts: 3
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How does new Truseq Stranded Protocol actually work? Is it dUTP based??
How does new Truseq Stranded Protocol actually work? Is it dUTP based??
I have not done this work. Please advise. thank you very much. |
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10-01-2012, 01:35 PM
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#2 |
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Senior Member
Location: Purdue University, West Lafayette, Indiana Join Date: Aug 2008
Posts: 2,317
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Yes it is dUTP based. Also adds actinomycin D to first strand synthesis, presumably to prevent any second strand synthesis during that step.
-- Phillip |
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10-01-2012, 03:24 PM
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#3 |
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Junior Member
Location: sf bay area Join Date: Oct 2010
Posts: 3
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Philip, Thank you very much clarifying this. Did you use this in your laboratory? and how happy are you with this?
thank you. |
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10-02-2012, 04:52 AM
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#4 |
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Senior Member
Location: Purdue University, West Lafayette, Indiana Join Date: Aug 2008
Posts: 2,317
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Have a kit, did not try it yet. Next week, maybe.
-- Phillip |
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