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02-15-2013, 12:04 PM
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#1 |
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Junior Member
Location: New York Join Date: Feb 2013
Posts: 2
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Qubit vs Nanodrop discrepancies??
Hi all,
What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit? Our core facility has generated Qubit values for my cDNA sampels that are consistently 1.3- to 2.3-fold higher than the Nanodrop values that I generated. I've read a lot of reports claiming that Nanodrop consistently overestimates concentration, so I'm a little puzzled by these Qubit results. Any help is greatly appreciated! Thanks in advance, Amy |
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07-10-2013, 08:50 AM
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#2 |
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Junior Member
Location: Santiago-Chile Join Date: Jul 2013
Posts: 1
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I have this same problem with my samples!! you managed to solve your doubts? please if your answer is positive, I discuss how? I have this same problem and I think that may be the differences in temperature between rooms!!
thank you |
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| Tags |
| cdna, dsdna, nanodrop, quantification, qubit |
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