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Old 10-07-2013, 10:24 AM   #1
Benny Chain
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Location: UCL, London

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Default Magnetic bead washers

Does anyone know of a automated bead washing machine which will efficiently work with the Ampure purification beads. I don't necessarily want to buy a whole robotics solution : I just would like to mechanise the repeated bead washes in our protocols, to reduce human error and pipetting time. Many thanks for any suggestions
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Old 10-08-2013, 01:20 AM   #2
cvhove
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hoi benny,

we have adapted a bead purification protocol on the Biomek 3000 platform and have to admit that this works well.
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Old 10-09-2013, 01:16 PM   #3
Chriswinefield
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Hi Benny,

we have just purchased the following item which might suite your needs - we are in the same boat, needing a cheap but effective alternative to a full robotics solution.
http://www.vp-scientific.com/Magneti..._Extractor.php

basically works on a 96 well format with 96 finger magnets. You pick up the beads and transfer to a new plate with wash buffer in etc. This will be very fast, with minimal liquid carry over and maximum retention of beads.
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Old 10-09-2013, 02:03 PM   #4
bstamps
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Could you share how much it was? I'm curious how it compares to the LifeTechnologies magnets.
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Old 10-09-2013, 02:25 PM   #5
chadn737
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Quote:
Originally Posted by Chriswinefield View Post
Hi Benny,

we have just purchased the following item which might suite your needs - we are in the same boat, needing a cheap but effective alternative to a full robotics solution.
http://www.vp-scientific.com/Magneti..._Extractor.php

basically works on a 96 well format with 96 finger magnets. You pick up the beads and transfer to a new plate with wash buffer in etc. This will be very fast, with minimal liquid carry over and maximum retention of beads.
I would also be interested in knowing how much this cost you.
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Old 10-09-2013, 02:29 PM   #6
Chriswinefield
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Not a problem - US$2,537.83. I'm not sure how this compares to the Life Technologies offering. As far as I am aware they don't offer a magnet in this configuration, rather they will have a magnetic base that the plate sits on and you then have to move the liquid - this operates the opposite way in that you capture and move the beads.

We are looking to couple this to a Machinary Nagal Plant DNA kit and the tech folk have said it should work well. As I understand it this format of magnetic cpature is used in the kingFisher nucleic acid prep robot.

hope this helps

PS there is a video on the website that shows how the beast works
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