Magnetic bead washers

Benny Chain · 10-07-2013, 10:24 AM

Does anyone know of a automated bead washing machine which will efficiently work with the Ampure purification beads. I don't necessarily want to buy a whole robotics solution : I just would like to mechanise the repeated bead washes in our protocols, to reduce human error and pipetting time. Many thanks for any suggestions

cvhove · 10-08-2013, 01:20 AM

hoi benny,

we have adapted a bead purification protocol on the Biomek 3000 platform and have to admit that this works well.

Chriswinefield · 10-09-2013, 01:16 PM

Hi Benny,

we have just purchased the following item which might suite your needs - we are in the same boat, needing a cheap but effective alternative to a full robotics solution.
http://www.vp-scientific.com/Magneti..._Extractor.php

basically works on a 96 well format with 96 finger magnets. You pick up the beads and transfer to a new plate with wash buffer in etc. This will be very fast, with minimal liquid carry over and maximum retention of beads.

bstamps · 10-09-2013, 02:03 PM

Could you share how much it was? I'm curious how it compares to the LifeTechnologies magnets.

chadn737 · 10-09-2013, 02:25 PM

Quote:

Originally Posted by Chriswinefield

Hi Benny,

we have just purchased the following item which might suite your needs - we are in the same boat, needing a cheap but effective alternative to a full robotics solution.
http://www.vp-scientific.com/Magneti..._Extractor.php

basically works on a 96 well format with 96 finger magnets. You pick up the beads and transfer to a new plate with wash buffer in etc. This will be very fast, with minimal liquid carry over and maximum retention of beads.

I would also be interested in knowing how much this cost you.

Chriswinefield · 10-09-2013, 02:29 PM

Not a problem - US$2,537.83. I'm not sure how this compares to the Life Technologies offering. As far as I am aware they don't offer a magnet in this configuration, rather they will have a magnetic base that the plate sits on and you then have to move the liquid - this operates the opposite way in that you capture and move the beads.

We are looking to couple this to a Machinary Nagal Plant DNA kit and the tech folk have said it should work well. As I understand it this format of magnetic cpature is used in the kingFisher nucleic acid prep robot.

hope this helps

PS there is a video on the website that shows how the beast works

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10-07-2013, 10:24 AM	#1
Benny Chain Junior Member Location: UCL, London Join Date: Dec 2011 Posts: 4	Magnetic bead washers Does anyone know of a automated bead washing machine which will efficiently work with the Ampure purification beads. I don't necessarily want to buy a whole robotics solution : I just would like to mechanise the repeated bead washes in our protocols, to reduce human error and pipetting time. Many thanks for any suggestions

10-08-2013, 01:20 AM	#2
cvhove Junior Member Location: Belgium Join Date: May 2011 Posts: 3	hoi benny, we have adapted a bead purification protocol on the Biomek 3000 platform and have to admit that this works well.

10-09-2013, 01:16 PM	#3
Chriswinefield Junior Member Location: Christchurch, New Zealand Join Date: Apr 2013 Posts: 2	Hi Benny, we have just purchased the following item which might suite your needs - we are in the same boat, needing a cheap but effective alternative to a full robotics solution. http://www.vp-scientific.com/Magneti..._Extractor.php basically works on a 96 well format with 96 finger magnets. You pick up the beads and transfer to a new plate with wash buffer in etc. This will be very fast, with minimal liquid carry over and maximum retention of beads.

10-09-2013, 02:03 PM	#4
bstamps Member Location: University of Oklaoma Join Date: Oct 2012 Posts: 40	Could you share how much it was? I'm curious how it compares to the LifeTechnologies magnets.

10-09-2013, 02:29 PM	#6
Chriswinefield Junior Member Location: Christchurch, New Zealand Join Date: Apr 2013 Posts: 2	Not a problem - US$2,537.83. I'm not sure how this compares to the Life Technologies offering. As far as I am aware they don't offer a magnet in this configuration, rather they will have a magnetic base that the plate sits on and you then have to move the liquid - this operates the opposite way in that you capture and move the beads. We are looking to couple this to a Machinary Nagal Plant DNA kit and the tech folk have said it should work well. As I understand it this format of magnetic cpature is used in the kingFisher nucleic acid prep robot. hope this helps PS there is a video on the website that shows how the beast works