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Old 01-13-2010, 09:15 AM   #1
runemoe
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Location: Oslo, Norway

Join Date: Jun 2009
Posts: 8
Default Empty constructs smallRNA

We are currently running a smallRNA project (standard protocol). We are having problems with empty constructs (e.g. dimers/trimers at approx. 90bp).
The correct gel bands which contain miRNAs/piRNAs are shown as 100 or even 110bp on the BioAnalyzer. According to our calculations the size of
all fragments should be 10bp less than they appear on the gel. Any other users sharing our problems?
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