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11-26-2013, 05:22 AM
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#1 |
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Member
Location: Brasil Join Date: Jun 2011
Posts: 12
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How to demultiplex NEXTERA EXOME RAPID DATA run in a Hiseq 2500
Please
Somebody could help me ? We are used to demultiplex Truseq data....but Nextera is not working ? Thanks |
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11-26-2013, 06:12 AM
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#2 |
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Senior Member
Location: London Join Date: Jun 2009
Posts: 298
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What do you mean by "not working"?
If you have access to the bcl files, you should be able to demultiplex using the CASAVA command configureBclToFastq.pl and a compatible spreadsheet http://supportres.illumina.com/docum..._15011196d.pdf |
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11-26-2013, 06:24 AM
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#3 |
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Senior Member
Location: East Coast USA Join Date: Feb 2008
Posts: 7,080
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Are you doing the de-multiplexing on the HiSeq itself or off-line using CASAVA?
You will have to provide some additional details in order for us to be able to help. |
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11-26-2013, 08:39 AM
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#4 | |
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Member
Location: Brasil Join Date: Jun 2011
Posts: 12
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Quote:
We are doing off-line using CASAVA Thank you very much |
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11-26-2013, 08:52 AM
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#5 |
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Senior Member
Location: East Coast USA Join Date: Feb 2008
Posts: 7,080
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CASAVA should not need any changes. What is exactly happening? Are you getting an error or is everything going into the "Undetermined" bin?
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