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Old 02-10-2014, 03:26 AM   #1
ymc
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Location: Hong Kong

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Default How does insert size affect downstream bioinfo analysis?

This is extracted from a BGI doc:

http://bgiamericas.com/wp-content/up...-2012.8.22.pdf
=================================
4. What insert size of FFPE samples and fresh frozen (FF) samples is used for library construction?

Based on our project experience, sizes are:
DNA FFPE samples: about 100bp
DNA FF samples: about 120bp
RNA FFPE samples: about 150bp
RNA FF samples: about 200bp
================================

Does knowing this affect downstream analysis? How do these numbers relate to fragment size?

Thanks in advance.
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