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02-10-2014, 03:26 AM
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Senior Member
Location: Hong Kong Join Date: Mar 2010
Posts: 498
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How does insert size affect downstream bioinfo analysis?
This is extracted from a BGI doc:
http://bgiamericas.com/wp-content/up...-2012.8.22.pdf ================================= 4. What insert size of FFPE samples and fresh frozen (FF) samples is used for library construction? Based on our project experience, sizes are: • DNA FFPE samples: about 100bp • DNA FF samples: about 120bp • RNA FFPE samples: about 150bp • RNA FF samples: about 200bp ================================ Does knowing this affect downstream analysis? How do these numbers relate to fragment size? Thanks in advance. |
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