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Old 06-05-2014, 09:25 AM   #1
Avro1986
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Location: Montreal

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Default Next-generation tutorial

Hi everyone!

I am a new biochemistry graduate student. I have started working with next-generation sequencing. During my undergraduate studies, I learned the Sanger method very well but not the former. I have looked at google and youtube for starting tutorials (even Illumina), but I am still confused. For example, I am confused with adaptors.

Does anyone know of an online tutorial video?

Thank you very much for your time.
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Old 06-05-2014, 10:07 AM   #2
blakeoft
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Someone posted a thread about a course on https://www.edx.org/ called PH525x Data Analysis for Genomics. You should still be able to sign up for it. If you do, then watching the "Next Generation Sequencing Technologies" lectures in week 2 will probably give you a good idea about what's going on. If I recall correctly, the videos give a bit more info than your typical intro that's on YouTube.

As far as info about adaptors, it doesn't go into an extremely rigorous explanation, but I think it's good enough to get the idea from what I remember.
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Old 06-05-2014, 10:26 AM   #3
GenoMax
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Quote:
Originally Posted by Avro1986 View Post
but I am still confused. For example, I am confused with adaptors.

Does anyone know of an online tutorial video?
Not a video but a PDF: http://tucf-genomics.tufts.edu/docum...q_Adapters.pdf
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Old 06-05-2014, 05:52 PM   #4
bilyl
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Quote:
Originally Posted by Avro1986 View Post
Hi everyone!

I am a new biochemistry graduate student. I have started working with next-generation sequencing. During my undergraduate studies, I learned the Sanger method very well but not the former. I have looked at google and youtube for starting tutorials (even Illumina), but I am still confused. For example, I am confused with adaptors.

Does anyone know of an online tutorial video?

Thank you very much for your time.
This one is a little dated but virtually everything is still the same:

https://www.broadinstitute.org/scien...yzer-boot-camp
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