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04-03-2015, 06:54 AM
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Junior Member
Location: Paris Join Date: Apr 2015
Posts: 1
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TruSeq Stranded total RNA - Cooling block passive for synthesizing 1st strand cDNA
Dear all,
I start my 1st automated experiment with TruSeq stranded total RNA. Unfortunately, my pipetting platform doesn't have a cooling block (I didn't choose this option when I bought it), my question is : - For the "Synthesize 1st strand cDNA" and "Adenylate 3' Ends" steps, basically, we need to incubate samples at 70°C then hold at 4°C. Can I process directly the next step without this 4°C, or use a cooling block passive (not controled temperature, so the temperature can be 4, 6, 10°C...)? Does anyone faced this problem? Help me please. Many thanks, |
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