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Old 12-10-2010, 01:41 PM   #1
PFS
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Default transcript length bias in enrichment analysis and RPKM

I read about the need of correcting for transcript length bias when performing GO enrichment analysis in RNA-Seq data.

If we use RPKM values instead of raw counts, is this still true?
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Old 12-12-2010, 06:32 PM   #2
frozenlyse
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No - check out this reference which goes through the reasoning and tries to address the problem though http://genomebiology.com/content/11/2/R14
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