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Old 03-09-2012, 11:56 AM   #1
Dr. Hybe
Location: Cambridge

Join Date: Jun 2009
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Default Can a MiSeq be used as a Cbot?

Would it be possible to perform cluster amplification and primer hyb. on the Miseq and then transfer the flowcell onto the HiSeq for sequencing? Just curious in the event your cBot were occupied. Thanks Dr. H
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Old 03-09-2012, 12:19 PM   #2
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The MiSeq can only cluster MiSeq flow cells. The flow cells are nothing alike in size nor connections to lane/lanes.

The HiSeq 2500 is supposed to be able to cluster flow cells on the instrument, bypassing the cBot.
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Old 03-10-2012, 10:25 AM   #3
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But only for "fast chemistry". The high output (old) chemistry will still require a cBot to cluster.

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