Newbie: Primer with index

tickteng · 04-23-2019, 06:26 PM

Hi everyone, kinda new to the whole sequencing. So I want to run multiple sample in minion. There is barcode provided by oxford nanopore, but i also would like to attach index to my pcr.

I am planning to make 18 or 22 bp of index.
The question is, is there any platform that you use to design index? or is it just randomly decided by you?

Marc_Jones · 04-24-2019, 12:53 AM

You could just add the index to the primers when you order them.

or

SQK-RPB004 or SQK-PBK004 depending on your experiment.

tickteng · 04-24-2019, 01:18 AM

hmm, not really like that. So beside the one provided by nanopore, I plan to attach some kind of index to the primer itself, so the result can be divided further in one barcode.

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04-23-2019, 06:26 PM	#1
tickteng Junior Member Location: Japan Join Date: Apr 2019 Posts: 6	Newbie: Primer with index Hi everyone, kinda new to the whole sequencing. So I want to run multiple sample in minion. There is barcode provided by oxford nanopore, but i also would like to attach index to my pcr. I am planning to make 18 or 22 bp of index. The question is, is there any platform that you use to design index? or is it just randomly decided by you?

04-24-2019, 12:53 AM	#2
Marc_Jones Member Location: UK Join Date: Jun 2017 Posts: 10	You could just add the index to the primers when you order them. or SQK-RPB004 or SQK-PBK004 depending on your experiment.

04-24-2019, 01:18 AM	#3
tickteng Junior Member Location: Japan Join Date: Apr 2019 Posts: 6	hmm, not really like that. So beside the one provided by nanopore, I plan to attach some kind of index to the primer itself, so the result can be divided further in one barcode.