SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
How to demultiplex NEXTERA EXOME RAPID DATA run in a Hiseq 2500 frossit Illumina/Solexa 4 11-26-2013 08:52 AM
Demultiplex amplicon data on duel barcodes and priming sequence. JackieBadger Bioinformatics 4 11-02-2012 06:49 AM
BCL to FASTQ conversation for paired-end RNA data seqmonkey Illumina/Solexa 8 09-27-2011 12:02 PM

Reply
 
Thread Tools
Old 09-14-2020, 12:20 AM   #1
frymor
Senior Member
 
Location: Germany

Join Date: May 2010
Posts: 150
Question demultiplex BCL data with two different barcoding systems

Hi,

I was wondering if anyone has experience in demultiplexing BCL files from Illumina with two different barcoding systems in one go?

we would like to know whether it is possible to use different, barcoded Tn5 adaptors during tagmentation and then use those Barcodes in combination with the barcodes on the sequencing primers to demultiplex the samples.

Has anyone has experience with this kind of analysis?
Is it possible to only add the Tn5 sequence to the primer sequence before demultiplexing and use it in the BCL2fastq run?

thanks
frymor is offline   Reply With Quote
Reply

Tags
demultiplexing, library perparation, primer, tagmentation

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 10:42 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO