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Old 06-07-2011, 11:17 AM   #1
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Location: USA

Join Date: Jun 2011
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Default FFPE DNA prep from LCM


Does anyone have experience, + or -, working with any commercial kits to take LCM'd FFPE tissue to Shearing step (Covaris) for SOLiD?
PamPetrel is offline   Reply With Quote
Old 06-08-2011, 12:41 AM   #2
Location: Leeds, UK

Join Date: Apr 2010
Posts: 63

We've used the qiagen qiaamp dna micro kit with reasonable results. For illumina rather than solid, so I don't know whether that would make a difference. It's a bit unpredictable. Mostly library prep works but sometimes it doesn't.
henry.wood is offline   Reply With Quote

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