Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
TruSeq or Nextera kit for library prep? rooty Sample Prep / Library Generation 12 10-29-2013 06:02 AM
Nextera vs Illumina Library Prep maggie Illumina/Solexa 6 03-01-2012 06:01 PM
Nextera Problem: Single adapters in PE500 library?? Kitty Sample Prep / Library Generation 14 07-26-2011 03:09 AM
Nextera vs Illumina Library Prep maggie Illumina/Solexa 2 12-30-2010 09:58 AM
Quantify Library before Cluster Generation kwebb Illumina/Solexa 7 06-22-2009 05:01 PM

Thread Tools
Old 12-14-2011, 12:26 AM   #1
Junior Member
Location: Ireland

Join Date: Sep 2011
Posts: 2
Default qPCR to validate/quantify Nextera library


I wish to validate my Nextera DNA library by qPCR.

Is this done merely by comparing against a control DNA library, i.e. the one that comes with the kit?

Is it possible to quanitify in absolute terms by qPCR or is this done with the BioAnalyzer?


mikmz is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 04:52 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO