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  • Homemade illumina oligos and library prep kit

    Hi,

    I am wondering if we can use the PCR amplification primers which were made at our side.
    In theory, this can work properly.

    But I am worrying a little bit if there can be some problem with sequencing primer binding and PCR amplification step.

    Is there anyone who attempt this?

    Thank you in advance.

  • #2
    Yes, as long as the sequences you use are the same as the Illumina sequences, primers you synthesize yourself work fine!

    Comment


    • #3
      Is this also true for adaptors and sequencing primers?

      Thanks
      Andi

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      • #4
        For the PE PCR primers, is it necessary to PAGE purify them?

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