Which are the best ITS primers to use for sequencing of environmental samples? Illumina suggests primers for 16S in the protocol but there aren't any suggestions for ITS sequencing. I've seen people using many different sets in literature e.g. ITSF1-ITS2, ITS1-ITS2, ITS3-ITS4. Moreover, is multiplexing primers i.e. 16S and ITS for the same sequencing run, a factor that could affect the selection of the ITS primers? or is it considered a bit "messy" to combine these two genes in one run?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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